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Characterization of HSD-P@V and in vitro evaluation on HSD and VNs. (A) Schematic illustration for the preparation and transformation of HSD-P@V. (B-D) TEM images of VNs (B), HSD-P@V (C) and HSD-P@V after incubation in PBS (pH 6.8) containing FAP-α (D). (E) XRD analysis of VNs and Val powder. (F) Val and (G) DOX release profiles under different conditions. Data are shown as the mean values ± SD ( n = 3). (H) Fluorescence distribution of collagen I <t>and</t> <t>α-SMA</t> in MCSs after incubation with different formulations for 72 h. (I) Fluorescence distribution of MCSs after incubation with NR, VNs/NR, HSD/NR, HSD-D@V/NR and HSD-P@V/NR for 12 h (MCSs in each group was pretreated with corresponding non-fluorescent labeling formulations for 72 h before co-incubation with NR labeling formulation).
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Characterization of HSD-P@V and in vitro evaluation on HSD and VNs. (A) Schematic illustration for the preparation and transformation of HSD-P@V. (B-D) TEM images of VNs (B), HSD-P@V (C) and HSD-P@V after incubation in PBS (pH 6.8) containing FAP-α (D). (E) XRD analysis of VNs and Val powder. (F) Val and (G) DOX release profiles under different conditions. Data are shown as the mean values ± SD ( n = 3). (H) Fluorescence distribution of collagen I and α-SMA in MCSs after incubation with different formulations for 72 h. (I) Fluorescence distribution of MCSs after incubation with NR, VNs/NR, HSD/NR, HSD-D@V/NR and HSD-P@V/NR for 12 h (MCSs in each group was pretreated with corresponding non-fluorescent labeling formulations for 72 h before co-incubation with NR labeling formulation).

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy

doi: 10.1016/j.ajps.2024.100888

Figure Lengend Snippet: Characterization of HSD-P@V and in vitro evaluation on HSD and VNs. (A) Schematic illustration for the preparation and transformation of HSD-P@V. (B-D) TEM images of VNs (B), HSD-P@V (C) and HSD-P@V after incubation in PBS (pH 6.8) containing FAP-α (D). (E) XRD analysis of VNs and Val powder. (F) Val and (G) DOX release profiles under different conditions. Data are shown as the mean values ± SD ( n = 3). (H) Fluorescence distribution of collagen I and α-SMA in MCSs after incubation with different formulations for 72 h. (I) Fluorescence distribution of MCSs after incubation with NR, VNs/NR, HSD/NR, HSD-D@V/NR and HSD-P@V/NR for 12 h (MCSs in each group was pretreated with corresponding non-fluorescent labeling formulations for 72 h before co-incubation with NR labeling formulation).

Article Snippet: Then, the protein on polyvinylidene difluoride (PVDF) membranes were incubated with anti-TGF-β (Bioss Co., bs-0086R) and anti-α-SMA primary antibody (Bioss Co., bs-10196R) at 4 °C overnight and then incubated with secondary antibody at room temperature for 1 h. Finally, the protein bands were observed by ECL reagent.

Techniques: In Vitro, Transformation Assay, Incubation, Fluorescence, Labeling, Formulation

In vivo anti-tumor mechanism of HSD-P@V. (A) Expression of TGF-β and α-SMA in tumor tissues by western blotting and semi-quantitative analysis. (B) The expression of collagen I and α-SMA in tumor tissues by immunofluorescence. (C, D) Senescence β-galactosidase staining images of tumor tissues (C) after the second dose and (D) at the end of treatment. (E) The expression of VCAM-1 in tumor tissues by immunofluorescence. (F) ELISA analysis of IL-15, IL-6, TNF-α and IFN-γ after treatment. All data are shown as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001.

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy

doi: 10.1016/j.ajps.2024.100888

Figure Lengend Snippet: In vivo anti-tumor mechanism of HSD-P@V. (A) Expression of TGF-β and α-SMA in tumor tissues by western blotting and semi-quantitative analysis. (B) The expression of collagen I and α-SMA in tumor tissues by immunofluorescence. (C, D) Senescence β-galactosidase staining images of tumor tissues (C) after the second dose and (D) at the end of treatment. (E) The expression of VCAM-1 in tumor tissues by immunofluorescence. (F) ELISA analysis of IL-15, IL-6, TNF-α and IFN-γ after treatment. All data are shown as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Then, the protein on polyvinylidene difluoride (PVDF) membranes were incubated with anti-TGF-β (Bioss Co., bs-0086R) and anti-α-SMA primary antibody (Bioss Co., bs-10196R) at 4 °C overnight and then incubated with secondary antibody at room temperature for 1 h. Finally, the protein bands were observed by ECL reagent.

Techniques: In Vivo, Expressing, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay